Kashino, Y.

To send e-mail, please paste @sci.u-hyogo.ac.jp after the mail-account above.

[English| Japanese ]

Advanced Low Carbon Technology Research and Development program -ALCA-

Generation of diatom factory through physiolomics toward a novel energy source

Research Interests


Biochemistry of Photosystem II

The photosynthetic physiology of ice algae and diatoms.
National Institute of Polar Research
Co-work for the photosynthetic physiology of ice algae and diatoms.

North Water Polynya Research Project
The International North Water Polynya Study (NOW), which is part of the International Arctic Polynya Programme (IAPP) of the Arctic Ocean Science Board (AOSB)

CASES project
the Canadian Arctic Shelf Exchange Study

Membership of academic societies:

The Japanese Society of Plant Physiologists
The Botanical Society of Japan
The Japanese Association for Photosynthesis Reserch

American Society of Plant Physiology
American Chemical Society
International Society of Photosynthesis Research

Recent Publication

Tokushima H, Inoue-Kashino N, Nakazato Y, Masuda A, Ifuku K & Kashino
Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer.
Biotechnol Biofuels 9: 235 (2016).
Impact Factor; 6.444

Fukunaga Y, Nakajima E, Hatano E, Itoh S, Kashino Y, Miyazawa A
Activation of NMDA receptors thickens the postsynaptic density via proteolysis.
Neurosci Res 101: 6-14 (2015).

Ishihara T, Ifuku K, Yamashita E, Fukunaga Y, Nishino Y, Miyazawa A, Kashino Y, Inoue-Kashino N
Utilization of light by fucoxanthin-chlorophyll-binding protein in a centric diatom, Chaetoceros gracilis.
Photosynth Res 126: 437-447 (2015).

Ifuku K, Yan D, Miyahara M, Inoue-Kashino N, Yamamoto YY & Kashino Y
A stable and efficient nuclear transformation system for the diatom Chaetoceros gracilis.
Photosynth Res 123: 203-211 (2014).

Kosugi M, Shizuma R, Moriyama Y, Koike H, Fukunaga Y, Takeuchi A, Uesugi K, Suzuki Y, Imura S, Kudoh S, Miyazawa A, Kashino Y & Kazuhiko S
Ideal osmotic spaces for chlorobionts or cyanobionts are differentially realized by lichenized fungi.
Plant Physiol 166: 337-348 (2014).

Aoi M, Kashino Y, Sato F & Ifuku K
Function and association of CyanoP in photosystem II of Synechocystis sp. PCC 6803.
"Photosynthesis and Artificial Photosynthesis Research" in Res Chem Intermed 40: 3209-3217 (2013).

Islam MR, Watanabe K, Kashino Y, Satoh K & Koike H
Spectral properties of a divinyl chlorophyll a harboring mutant of Synechocystis sp. PCC6803.
Photosynth Res 117: 245-255 (2013).

Kosugi M, Miyake H, Yamakawa H, Shibata Y, Miyazawa A, Sugimura T, Satoh K, Itoh S & Kashino Y
Arabitol provided by lichenous fungi enhances ability to dissipate excess light energy in a symbiotic green alga under desiccation.
Plant Cell Physiolo 54: 1316-1325 (2013).

Miyahara M, Aoi M, Inoue-Kashino N, Kashino Y & Ifuku K
Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation.
Biosci Biotechnol Biochem 77: 120936-1-3 (2013).

Kosugi M, Kashino Y, Kudoh S & Imura S
Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll cenrtifugation (in Japanese).
Antarctic Record 56: 285-293 (2013).

Ozawa S, Kosugi M, Kashino Y, Sugimura T & Takahashi Y
5'-Monohydroxyphylloquinone is the dominant naphthoquinone of PSI in the green alga Chlamydomonas reinhardtii.
Plant Cell Physiolo 53: 237-243 (2012).

Sekine F, Horiguchi K, Kashino Y, Shimizu Y, Yu L-J, Kobayashi M & Wang Z-Y
Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum.
Photosynth Res 111: 9-18 (2011).

Miyake H, Komura M, Itoh S, Kosugi M, Kashino Y, Satoh K & Shibata Y
Multiple dissipation components of excess light energy in dry lichen revealed by ultrafast fluorescence study at 5 K.
Photosynth Res 110: 39-48 (2011).

Inoue-Kashino N, Kashino Y and Takahashi Y
Psb30 is a photosystem II reaction center subunit and is required for optimal growth in high light in Chlamydomonas reinhardtii.
J Photochem Photobiol B: Biology: 220-228 (2011).

Tanabe Y, Shitara T, Kashino Y, Hara Y and Kudoh S
Utilizing the effective xanthophylls cycle for blooming of Ochromonas smithii and O. itoi (Chrysophyceae) on the snow surface.
PloS ONE 6: e14690 (2011).

Inoue-Kashino N, Kashino Y, Orii H, Satoh K, Terashima I and Pakrasi HB
S4 Protein Sll1252 Is Necessary for Energy Balancing in Photosynthetic Electron Transport in Synechocystis sp. PCC 6803.
Biochemistry 50: 329-339 (2011).

Kogo N, Tazaki A, Kashino Y, Morichika K, Orii H, Mochii M and Watanabe K
Gern-line mitochondria exhibit suppressed respiratory activity to support their accurate transmission to the next generation.
Developmental Biology 349: 462-469 (2011).

Hashihama F, Umeda H, Hamada C, Kudoh S, Hirawake T, Satoh K, Fukuchi M and Kashino Y
Light acclimation states of phytoplankton in the Southern Ocean, determined using photosynthetic pigment distribution.
Mar Biol 157: 2263-2278 (2010).

Sugiura M, Harada S, Manabe, Hayashi, Kashino Y and Boussac A
Psb30 contributes to structurally stabilise the Photosystem II complex in the thermophilic cyanobacterium Thermosynehcococcus elongatus.
Biochim Biophys Acta 1797: 1546-1554 (2010).

Kosugi M, Katashima Y, Aikawa S, Tanabe Y, Kudoh S, Kashino Y, Koike H and Satoh K
Comparative study on the photosynthetic properties of Prasiola (Chlorophyceae) and Nostoc (Cyanophyceae) from Antarctic and non-Antarctic sites.
J Phycol 46: 466-476 (2010).

Kosugi M, Kashino Y and Satoh K
Comparative analysis of light curves of Ramalina yasudae and freshly isolated Trebouxia sp. revealed the presence of intrinsic protection mechanisms independent of upper cortex for the photosynthetic system of algal symbionts in lichen.
Lichenology 9: 1-10 (2010).

Nagao R, Tomo T, Noguchi E, Nakajima S, Suzuki T, Okumura A, Kashino Y, Mimuro M, Ikeuchi M and Enami I
Purification and characterization of a stable oxygen-evolving Photosystem II complex from a marine centric diatom, Chaetoceros gracilis.
Biochim Biophys Acta 1797: 160-166 (2010)

Ikeya T, Horimoto N and Kashino Y
A practical method for sensitive determination of the fluorescent water-tracer uranine by reversed phase HPLC under alkaline conditions.
Talanta 79: 818-823 (2009).

Takahashi T, Inoue-Kashino N, Ozawa S, Takahashi Y, Kashino Y, and Satoh K
Photosystem II complex in vivo is a monomer.
J Biol Chem 284: 15598-15606 (2009).

Kosugi M, Arita M, Shizuma R, Moriyama Y, Kashino Y, Koike H, and Satoh K
Responses to desiccation stress in lichens are different from those in their photobionts.
Plant Cell Physiol 50: 879-888 (2009).

Aikawa S, Hattori H, Gomi Y, Watanabe K, Kudoh S, Kashino Y, and Satoh K
Diel tuning of photosynthetic systems in ice algae at Saroma-ko Lagoon, Hokkaido, Japan.
Polar Sci 3: 57-72 (2009).

Ooyabu J, Ohtsuka M, Kashino Y, Koike H, and Satoh K
The expression pattern of NAD(P)H oxidases and the cyclic electron transport pathway around photosystem I of Synechocystis sp. PCC6803 depend on growth conditions.
Biosci Biotechnol Biochem 72: 3180-3188 (2008).

Inoue-Kashino N, Takahashi T, Ban A, Sugiura M, Takahashi Y, Satoh K and Kashino Y
Evidence for a stable association of Psb30 (Ycf12) with photosystem II core complex in the cyanobacterium Synechocystis sp. PCC 6803.
Photosynth Res: 323-335 (2008).

Islam MR, Aikawa S, Midorikawa T, Kashino Y, Satoh K and Koike H
slr1923 of Synechocystis sp. PCC 6803 is essential for conversion of 3,8-divinyl(proto)chlorophyll(ide) to 3-monovinyl(proto)chlorophyll(ide).
Plant Physiol 148: 1068-1081 (2008).

Yamori W, Noguchi K, Kashino Y, and Terashima I
The role of the electron transport in determining the temperature dependence of photosynthetic rate in spinach leaves grown at contrasting temperatures.
Plant Cell Physiol 49: 583 - 591 (2008).

Ikeda Y, Komura M, Watanabe M, Minami C, Koike H, Itoh S, Kashino Y and Satoh K
Photosystem I complexes associated with fucoxanthin-chlorophyll-binding proteins from a marine centric diatom, Chaetoceros gracilis.
Biochim Biophys Acta 1777: 351-361 (2008).

Nagao R, Ishii A, Tada O, Suzuki T, Dohmae N, Okumura A, Iwai M, Takahashi T, Kashino Y and Enami I
Isolation and characterization of oxygen-evolving thylakoid membranes and Photosystem II particles from a marine diatom Chaetoceros gracilis.
Biochim Biophys Acta 1767: 1353-1362 (2007).

Kashino Y, Takahashi T, Inoue-Kashino N, Ban A, Ikeda Y, Satoh K and Sugiura M
Ycf12 is a core subunit in the photosystem II complex.
Biochim Biophys Acta 1767(11): 1269-1275 (2007).

Kashino Y, Harayama T, Pakrasi HB and Satoh K
Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis.
J Chromatogr B 849: 282-292 (2007).

Roose JL, Kashino Y and Pakrasi HB
The PsbQ protein defines cyanobacterial photosystem II complexes with highest activity and stability.
Proc Natl Acad Sci USA 104:.2548-2553 (2007).

Ohnishi N, Kashino Y, Satoh K, Ozawa S and Takahashi Y
Chloroplast-encoded polypeptide PsbT is involved in the repair of primary electron acceptor QA of photosystem II during photoinhibition in Chlamydomonas reinhardtii.
J Biol Chem 282: 7107-7115 (2007).

Kashino Y, Inoue-Kashino N, Roose JL & Pakrasi HB
Absence of the PsbQ Protein Results in Destabilization of the PsbV Protein and Decreased Oxygen Evolution Activity in Cyanobacterial Photosystem II.
J Biol Chem, Jul 2006; 281: 20834-20841 (2006).

Ban A, Aikawa S, Hattori H, Sasaki H, Sampei M, Kudoh S, Fukuchi M, Satoh K & Kashino Y
Comparative analysis of photosynthetic properties in ice algae and phytoplankton inhabiting Franklin Bay, the Canadian Arctic, with those in mesophilic diatoms during CASES 03-04.
Polar Bioscience 19; 11-28 (2006).

Inoue-Kashino N, Kashino Y, Satoh K, Terashima I & Pakrasi HB
PsbU Provides a Stable Architecture for the Oxygen-Evolving System in Cyanobacterial Photosystem II
Biochemistry 44(36): 12214 - 12228 (2005).

Thornton LE., Ohkawa H, Roose JL, Kashino Y, Keren N & Pakrasi HB
Homologs of Plant PsbP and PsbQ Proteins Are Necessary for Regulation of Photosystem II Activity in the Cyanobacterium Synechocystis 6803
Plant Cell 16: 2164-2175 (2004)

Kashino Y
Separation methods in the analysis of protein membrane complexes.
Journal of Chromatography B 797 (1-2): 191-216 (2003)

Kashino Y & Kudoh S
Concerted response of xanthophyll-cycle pigments in a marine diatom, Chaetoceros gracilis, to the sifts of light condition.
Phycological Research 51 (3): 168-172 (2003)

Kudoh S, Imura S & Kashino Y
Xanthophyll cycle of ice algae prevailing in sea ice bottom in Saroma Ko lagoon, Hokkaido, Japan.
Polar Bioscience 16: 86-97 (2003)

Kudoh S, Kashino Y & Imura S
Ecological studies on aquatic moss pillars in Antarctic lakes. 3. Light response and chilling and heat sensitivity of photosynthesis.
Polar Bioscience 16: 33-42 (2003)

Kashino Y, Koike H, Yoshio M, Egashira H, Ikeuchi M, Pakrasi HB, Satoh K.
Low-molecular-mass polypeptide components of a photosystem II preparation from the thermophilic cyanobacterium Thermosynechococcus vulcanus.
Plant Cell Physiol 43(11):1366-73 (2002).

Kashino Y, Lauber WM, Carroll JA, Wang Q, Whitmarsh J, Satoh J & Pakrasi HB
Proteomic analysis of a highly active photosystem II preparation from the cyanobacterium Synechocystis sp. PCC 6803 reveals the presence of novel polypeptides.
Biochemistry 41(25):8004-12 (2002).

Kashino Y, Kudoh S, Hayashi Y, Suzuki Y, Odate T, Hirawake T, Satoh K & Fukuchi M
Strategies of phytoplankton to perform effective photosynthesis in the North Water.
Deep Sea Research II 49: 5049-5061 (2002).

Sampei, M., Sasaki, M., Hattori, H., Kudoh, S., Kashino, Y., Fukuchi, M.
Seasonal and spatial variability in the flux of biogenic particles in the North Water polynya in 1997-1998.
Deep Sea Research II 49: 5245-5257(2002)

Kashino Y, Koike H & Satoh K
An improved sodium dodecyl sulfate-polyacrylamide gel electrophoresis system for the analysis of membrane protein complexes.
Electrophoresis 22 (6):1004-7 (2001).

Ikeya T, Kashino Y, Kudoh S, Imura S, Watanabe K & Fukuchi M
Acclimation of photosynthetic properties in psychrophilic diatom isolated under different light intensities.
Polar Bioscience 13: 43-54 (2000).

Kashino Y, Fujimoto K, Akamatsu A, Koike H, Satoh K & Kudoh S.
Photosynthetic pigment composition of ice algal and phytoplankton assemblages in early spring in Saroma ko lagoon, Hokkaido, Japan.
Proc. NIPR Symp. Polar Biol., 11: pp.22-32 (1998)

Department of Life Science
Faculty of Science

If you need contact, please feel free to send e-mail to Kashino.
To send e-mail, please paste @sci.u-hyogo.ac.jp after the mail-account above.