Kashino, Y.
e-mail;kashino
To send e-mail, please paste @sci.u-hyogo.ac.jp after the mail-account above.
[English| Japanese ]
Research Interests
Biochemistry of Photosystem II
The photosynthetic physiology of ice algae and diatoms.
National Institute of Polar Research
Co-work for the photosynthetic physiology of ice algae and diatoms.
North Water Polynya Research Project
The International North Water Polynya Study (NOW), which is part of the International Arctic Polynya Programme (IAPP) of the Arctic Ocean Science Board (AOSB)
the Canadian Arctic Shelf Exchange Study
Membership of academic societies:
The Japanese Society of Plant Physiologists
The Botanical Society of Japan
The Japanese Association for Photosynthesis Reserch
American Society of Plant Physiology
American Chemical Society
International Society of Photosynthesis Research
Recent Publication
1. Kosugi M, Ohtani S, Hara K, Toyoda A, Nishide H, Ozawa S-I, Takahashi Y, Kashino Y, Kudoh S, Koike H and Minagawa J (2024) Characterization of the far-red light absorbing light-harvesting chlorophyll a/b binding complex, a derivative of the distinctive Lhca gene family in green algae. Front. Plant Sci. 15:1409116 (DOI 10.3389/fpls.2024.1409116).
2. Oishi K, Nagamori M, Kashino Y, Sekiguchi H, Sasaki YC, Miyazawa A, & Nishino Y (2023) Ligand-Dependent Intramolecular Motion of Native Nicotinic Acetylcholine Receptors Determined in Living Myotube Cells via Diffracted X-ray Tracking. Int. J. Mol. Sci. 24: 12069. (https://doi.org/10.3390/ijms241512069)
3. Toda N, Inoue-Kashino N, Fujita H, Yoshida R, Nimura-Matsune K, Watanabe S, Kuroda A, Kashino Y, & and Hirota R (2024) Cell morphology engineering enhances grazing resistance of Synechococcus elongatus PCC 7942 for non-sterile large-scale cultivation, J Biosci Bioeng 137(4): 245-253 (https://doi.org/10.1016/j.jbiosc.2024.01.001)
4. Kosugi M, Kawasaki M, Shibata Y, Hara K, Takaichi S, Moriya T, Adachi N, Kamei Y, Kashino Y, Kudoh S, Koike H & *Senda T (2023) Uphill energy transfer mechanism for photosynthesis in an Antarctic alga. Nature Communications 14:730 (https://doi.org/10.1038/s41467-023-36245-1)
5. *Kimura A, Kitoh-Nishioka H, Aota T, Hamaguchi T, Yonekura K, Kawakami K, Shinzawa-Itoh K, Inoue-Kashino N, Ifuku K, Yamashita E, Kashino Y & Itoh S (2022) Theoretical model of the far-red-light-adapted photosystem i reaction center of cyanobacterium Acaryochloris marina using chlorophyll d and the effect of chlorophyll exchange. J. Phys. Chem. B 126: 4009−4021 (https://doi.org/10.1021/acs.jpcb.2c00869)
6. Inoue H, Tajima K, Mitsumori C, Inoue-Kashino N, Miura T, Ifuku K, Hirota R, Kashino Y, Fujita K and *Kinoshita H (2022) Biodiversity risk assessment of genetically modified Chaetoceros gracilis for outdoor cultivation. J. Gen. Appl. Microbiol. 68(3): 151-162 (doi 10.2323/jgam.2021.11.001)
7. Joaquín-Ovalle FM, Guihurt G, Barcelo-Bovea V, Hani Saba A, Fontanet-Gómez NC, Ramirez-Paz J, Kashino Y, Doble Cacho K, Torres-Martinez Z, Delinois LJ, *Delgado Y & *Griebenow K (2022) Oxidative stress- and autophagy-inducing effects of PSI-LHCI from Botryococcus braunii in breast cancer cells BioTech 11: 9 (https://doi.org/10.3390/biotech11020009)
8. Kumazawa M, Nishide H, Inoue-Kashino N, Shen J-R, Nagao R, Nakano T, Uchiyama I, Kashino Y & *Ifuku K (2022) Molecular phylogeny of fucoxanthin-chlorophyll a/c proteins from Chaetoceros gracilis and Lhcq/Lhcf diversity. Physiol Plant 174: e13598. (https://doi.org/10.1111/ppl.13598)
*9. Hamaguchi T, Kawakami K, Shinzawa-Itoh K, Inoue-Kashino N, Itoh S, Ifuku K, Yamashita E, Maeda K, Yonekura K & *Kashino Y (2021) Structure of the far-red light utilizing photosystem I of Acaryochloris marina. Nat Commun 12: 2333 (https://doi.org/10.1038/s41467-021-22502-8)
10. Nagao R, Kato K, Ifuku K, Suzuki T, Kumazawa M, Uchiyama I, Kashino Y, Dohmae N, Akimoto S, Shen J-R, Miyazaki N & *Akita F (2020) Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex. Nat Commun 11: 2481- (https://doi.org/10.1038/s41467-020-16324-3)
11. Kawahara K, Inoue-Kahino N, Namie K, Kato Y, Tomo T, Shibata Y, Kashino Y & *Noguchi T (2020) A gold nanoparticle conjugate with photosystem I and photosystem II for development of a biohybrid water-splitting photocatalyst. Biomed Spectrosc Imaging 9: 73–81 (https://doi.org/10.3233/BSI-200200)
12. *Kosugi M, Ozawa S-I, Takahashi Y, Kamei Y, Itoh S, Sakae Kudoh S, Kashino Y, Koike H (2019) Red-shifted chlorophyll a bands allow uphill energy transfer to photosystem II reaction centers in an aerial green alga, Prasiola crispa, harvested in Antarctica, Biochim Biophys Acta 1861 (2): 148139 (https://doi.org/10.1016/j.bbabio.2019.148139)
13. Suzuki T, Nishizawa A, Kikuchi M, Nonaka C, Komuro M, Nakayama M, Kashino Y, Fukuda M & *Kimura S (2019) Biphenyl degradation by recombinant photosynthetic cyanobacterium Synechocystis sp. PCC6803 in an oligotrophic environment using unphysiological electron transfer, Biochem J 476 (23): 3615-3630 (https://doi.org/10.1042/BCJ20190731)
14. *Itoh K, Nakasuji T, Kashino Y, Ifuku K, Maeda K, Yamamoto T and Taguchi S (2019) Influence of air flow rate on flotation of marine diatoms using venturi tube type microbubble generator, Bull. Soc. Sea Water Sci., Jpn.: 73, 354 - 355. (https://doi.org/10.11457/swsj.73.6_354)
15. Nagao R, Kato K, Suzuki T, Ifuku K, Uchiyama I, Kashino Y, Dohmae N, Akimoto S, Shen J-R, Miyazaki N & *Akita F (2019) Structural basis for energy harvesting and dissipation in a diatom PSII–FCPII supercomplex, Nature Plants 5: 890–901. (https://doi.org/10.1038/s41477-019-0477-x)
16. *Itoh K, Kashino Y, Ifuku K, Kouji M, Yamamoto T, Taguchi S (2019) Dispersed air flotation of microalgae using venturi tube type microbubble generator. Biomass Bioenergy 130: 105379 published online (https://doi.org/10.1016/j.biombioe.2019.105379)
17. Endo Y, Hatanaka T, *Maeda K, Arafune K, Yamamoto T, Itoh K, Kuramochi H, Kashino Y & Ifuku K (2018) Use of ethanol with triolein for fatty acid ethyl ester as biodiesel fuel in a Novozym® 435 fixed-bed reactor. Biomass Bioenergy 108: 433-438 (https://doi.org/10.1016/j.biombioe.2017.11.023)
18. *Maeda K, Itoh K, Kashino Y, Ifuku K, Arafune K & Yamamoto T (2018) Crystallizing concentration of the diatom Chaetoceros gracilis cell solutions (in Japanese). Kagaku Kogaku Ronbunshu 44: 18-22. (https://doi.org/10.1252/kakoronbunshu.44.18)
19. *Kosugi M, Lee C, Misaki T, Fujita M, Kashino Y & Sugimura T (2017) Stereochemical assignment of a unique electron acceptor, polar analogue of vitamin K1 (5´-hydroxyphylloquinone), in Photosystem I. Biosci Biotechnol Biochem 72: 3180-3188.(https://doi.org/10.1080/09168451.2017.1385381)
Tokushima H, Inoue-Kashino N, Nakazato Y, Masuda A, Ifuku K & Kashino
Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer.
Biotechnol Biofuels 9: 235 (2016).
Impact Factor; 6.444
Fukunaga Y, Nakajima E, Hatano E, Itoh S, Kashino Y, Miyazawa A
Activation of NMDA receptors thickens the postsynaptic density via proteolysis.
Neurosci Res 101: 6-14 (2015).
Ishihara T, Ifuku K, Yamashita E, Fukunaga Y, Nishino Y, Miyazawa A, Kashino Y, Inoue-Kashino N
Utilization of light by fucoxanthin-chlorophyll-binding protein in a centric diatom, Chaetoceros gracilis.
Photosynth Res 126: 437-447 (2015).
Ifuku K, Yan D, Miyahara M, Inoue-Kashino N, Yamamoto YY & Kashino Y
A stable and efficient nuclear transformation system for the diatom Chaetoceros gracilis.
Photosynth Res 123: 203-211 (2014).
Ideal osmotic spaces for chlorobionts or cyanobionts are differentially realized by lichenized fungi.
Plant Physiol 166: 337-348 (2014).
Aoi M, Kashino Y, Sato F & Ifuku K
Function and association of CyanoP in photosystem II of Synechocystis sp. PCC 6803.
"Photosynthesis and Artificial Photosynthesis Research" in Res Chem Intermed 40: 3209-3217 (2013).
Islam MR, Watanabe K, Kashino Y, Satoh K & Koike H
Spectral properties of a divinyl chlorophyll a harboring mutant of Synechocystis sp. PCC6803.
Photosynth Res 117: 245-255 (2013).
Kosugi M, Miyake H, Yamakawa H, Shibata Y, Miyazawa A, Sugimura T, Satoh K, Itoh S & Kashino Y
Arabitol provided by lichenous fungi enhances ability to dissipate excess light energy in a symbiotic green alga under desiccation.
Plant Cell Physiolo 54: 1316-1325 (2013).
Miyahara M, Aoi M, Inoue-Kashino N, Kashino Y & Ifuku K
Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation.
Biosci Biotechnol Biochem 77: 120936-1-3 (2013).
Kosugi M, Kashino Y, Kudoh S & Imura S
Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll cenrtifugation (in Japanese).
Antarctic Record 56: 285-293 (2013).
Ozawa S, Kosugi M, Kashino Y, Sugimura T & Takahashi Y
5'-Monohydroxyphylloquinone is the dominant naphthoquinone of PSI in the green alga Chlamydomonas reinhardtii.
Plant Cell Physiolo 53: 237-243 (2012).
Sekine F, Horiguchi K, Kashino Y, Shimizu Y, Yu L-J, Kobayashi M & Wang Z-Y
Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum.
Photosynth Res 111: 9-18 (2011).
Miyake H, Komura M, Itoh S, Kosugi M, Kashino Y, Satoh K & Shibata Y
Multiple dissipation components of excess light energy in dry lichen revealed by ultrafast fluorescence study at 5 K.
Photosynth Res 110: 39-48 (2011).
Inoue-Kashino N, Kashino Y and Takahashi Y
Psb30 is a photosystem II reaction center subunit and is required for optimal growth in high light in Chlamydomonas reinhardtii.
J Photochem Photobiol B: Biology: 220-228 (2011).
Tanabe Y, Shitara T, Kashino Y, Hara Y and Kudoh S
Utilizing the effective xanthophylls cycle for blooming of Ochromonas smithii and O. itoi (Chrysophyceae) on the snow surface.
PloS ONE 6: e14690 (2011).
Inoue-Kashino N, Kashino Y, Orii H, Satoh K, Terashima I and Pakrasi HB
S4 Protein Sll1252 Is Necessary for Energy Balancing in Photosynthetic Electron Transport in Synechocystis sp. PCC 6803.
Biochemistry 50: 329-339 (2011).
Kogo N, Tazaki A, Kashino Y, Morichika K, Orii H, Mochii M and Watanabe K
Gern-line mitochondria exhibit suppressed respiratory activity to support their accurate transmission to the next generation.
Developmental Biology 349: 462-469 (2011).
Hashihama F, Umeda H, Hamada C, Kudoh S, Hirawake T, Satoh K, Fukuchi M and Kashino Y
Light acclimation states of phytoplankton in the Southern Ocean, determined using photosynthetic pigment distribution.
Mar Biol 157: 2263-2278 (2010).
Sugiura M, Harada S, Manabe, Hayashi, Kashino Y and Boussac A
Psb30 contributes to structurally stabilise the Photosystem II complex in the thermophilic cyanobacterium Thermosynehcococcus elongatus.
Biochim Biophys Acta 1797: 1546-1554 (2010).
Kosugi M, Katashima Y, Aikawa S, Tanabe Y, Kudoh S, Kashino Y, Koike H and Satoh K
Comparative study on the photosynthetic properties of Prasiola (Chlorophyceae) and Nostoc (Cyanophyceae) from Antarctic and non-Antarctic sites.
J Phycol 46: 466-476 (2010).
Kosugi M, Kashino Y and Satoh K
Comparative analysis of light curves of Ramalina yasudae and freshly isolated Trebouxia sp. revealed the presence of intrinsic protection mechanisms independent of upper cortex for the photosynthetic system of algal symbionts in lichen.
Lichenology 9: 1-10 (2010).
Purification and characterization of a stable oxygen-evolving Photosystem II complex from a marine centric diatom, Chaetoceros gracilis.
Biochim Biophys Acta 1797: 160-166 (2010)
Ikeya T, Horimoto N and Kashino Y
A practical method for sensitive determination of the fluorescent water-tracer uranine by reversed phase HPLC under alkaline conditions.
Talanta 79: 818-823 (2009).
Takahashi T, Inoue-Kashino N, Ozawa S, Takahashi Y, Kashino Y, and Satoh K
Photosystem II complex in vivo is a monomer.
J Biol Chem 284: 15598-15606 (2009).
Kosugi M, Arita M, Shizuma R, Moriyama Y, Kashino Y, Koike H, and Satoh K
Responses to desiccation stress in lichens are different from those in their photobionts.
Plant Cell Physiol 50: 879-888 (2009).
Aikawa S, Hattori H, Gomi Y, Watanabe K, Kudoh S, Kashino Y, and Satoh K
Diel tuning of photosynthetic systems in ice algae at Saroma-ko Lagoon, Hokkaido, Japan.
Polar Sci 3: 57-72 (2009).
Ooyabu J, Ohtsuka M, Kashino Y, Koike H, and Satoh K
The expression pattern of NAD(P)H oxidases and the cyclic electron transport pathway around photosystem I of Synechocystis sp. PCC6803 depend on growth conditions.
Biosci Biotechnol Biochem 72: 3180-3188 (2008).
Inoue-Kashino N, Takahashi T, Ban A, Sugiura M, Takahashi Y, Satoh K and Kashino Y
Evidence for a stable association of Psb30 (Ycf12) with photosystem II core complex in the cyanobacterium Synechocystis sp. PCC 6803.
Photosynth Res: 323-335 (2008).
Islam MR, Aikawa S, Midorikawa T, Kashino Y, Satoh K and Koike H
slr1923 of Synechocystis sp. PCC 6803 is essential for conversion of 3,8-divinyl(proto)chlorophyll(ide) to 3-monovinyl(proto)chlorophyll(ide).
Plant Physiol 148: 1068-1081 (2008).
Yamori W, Noguchi K, Kashino Y, and Terashima I
The role of the electron transport in determining the temperature dependence of photosynthetic rate in spinach leaves grown at contrasting temperatures.
Plant Cell Physiol 49: 583 - 591 (2008).
Ikeda Y, Komura M, Watanabe M, Minami C, Koike H, Itoh S, Kashino Y and Satoh K
Photosystem I complexes associated with fucoxanthin-chlorophyll-binding proteins from a marine centric diatom, Chaetoceros gracilis.
Biochim Biophys Acta 1777: 351-361 (2008).
Nagao R, Ishii A, Tada O, Suzuki T, Dohmae N, Okumura A, Iwai M, Takahashi T, Kashino Y and Enami I
Isolation and characterization of oxygen-evolving thylakoid membranes and Photosystem II particles from a marine diatom Chaetoceros gracilis.
Biochim Biophys Acta 1767: 1353-1362 (2007).
Kashino Y, Takahashi T, Inoue-Kashino N, Ban A, Ikeda Y, Satoh K and Sugiura M
Ycf12 is a core subunit in the photosystem II complex.
Biochim Biophys Acta 1767(11): 1269-1275 (2007).
Kashino Y, Harayama T, Pakrasi HB and Satoh K
Preparation of membrane proteins for analysis by two-dimensional gel electrophoresis.
J Chromatogr B 849: 282-292 (2007).
Roose JL, Kashino Y and Pakrasi HB
The PsbQ protein defines cyanobacterial photosystem II complexes with highest activity and stability.
Proc Natl Acad Sci USA 104:.2548-2553 (2007).
Ohnishi N, Kashino Y, Satoh K, Ozawa S and Takahashi Y
Chloroplast-encoded polypeptide PsbT is involved in the repair of primary electron acceptor QA of photosystem II during photoinhibition in Chlamydomonas reinhardtii.
J Biol Chem 282: 7107-7115 (2007).
Kashino Y, Inoue-Kashino N, Roose JL & Pakrasi HB
Absence of the PsbQ Protein Results in Destabilization of the PsbV Protein and Decreased Oxygen Evolution Activity in Cyanobacterial Photosystem II.
J Biol Chem, Jul 2006; 281: 20834-20841 (2006).
Ban A, Aikawa S, Hattori H, Sasaki H, Sampei M, Kudoh S, Fukuchi M, Satoh K & Kashino Y
Comparative analysis of photosynthetic properties in ice algae and phytoplankton inhabiting Franklin Bay, the Canadian Arctic, with those in mesophilic diatoms during CASES 03-04.
Polar Bioscience 19; 11-28 (2006).
Inoue-Kashino N, Kashino Y, Satoh K, Terashima I & Pakrasi HB
PsbU Provides a Stable Architecture for the Oxygen-Evolving System in Cyanobacterial Photosystem II
Biochemistry 44(36): 12214 - 12228 (2005).
Thornton LE., Ohkawa H, Roose JL, Kashino Y, Keren N & Pakrasi HB
Homologs of Plant PsbP and PsbQ Proteins Are Necessary for Regulation of Photosystem II Activity in the Cyanobacterium Synechocystis 6803
Plant Cell 16: 2164-2175 (2004)
Separation methods in the analysis of protein membrane complexes.
Journal of Chromatography B 797 (1-2): 191-216 (2003)
Concerted response of xanthophyll-cycle pigments in a marine diatom, Chaetoceros gracilis, to the sifts of light condition.
Phycological Research 51 (3): 168-172 (2003)
Kudoh S, Imura S & Kashino Y
Xanthophyll cycle of ice algae prevailing in sea ice bottom in Saroma Ko lagoon, Hokkaido, Japan.
Polar Bioscience 16: 86-97 (2003)
Kudoh S, Kashino Y & Imura S
Ecological studies on aquatic moss pillars in Antarctic lakes. 3. Light response and chilling and heat sensitivity of photosynthesis.
Polar Bioscience 16: 33-42 (2003)
Kashino Y, Koike H, Yoshio M, Egashira H, Ikeuchi M, Pakrasi HB, Satoh K.
Low-molecular-mass polypeptide components of a photosystem II preparation from the thermophilic cyanobacterium Thermosynechococcus vulcanus.
Plant Cell Physiol 43(11):1366-73 (2002).
Kashino Y, Lauber WM, Carroll JA, Wang Q, Whitmarsh J, Satoh J & Pakrasi HB
Proteomic analysis of a highly active photosystem II preparation from the cyanobacterium Synechocystis sp. PCC 6803 reveals the presence of novel polypeptides.
Biochemistry 41(25):8004-12 (2002).
Kashino Y, Kudoh S, Hayashi Y, Suzuki Y, Odate T, Hirawake T, Satoh K & Fukuchi M
Strategies of phytoplankton to perform effective photosynthesis in the North Water.
Deep Sea Research II 49: 5049-5061 (2002).
Sampei, M., Sasaki, M., Hattori, H., Kudoh, S., Kashino, Y., Fukuchi, M.
Seasonal and spatial variability in the flux of biogenic particles in the North Water polynya in 1997-1998.
Deep Sea Research II 49: 5245-5257(2002)
An improved sodium dodecyl sulfate-polyacrylamide gel electrophoresis system for the analysis of membrane protein complexes.
Electrophoresis 22 (6):1004-7 (2001).
Ikeya T, Kashino Y, Kudoh S, Imura S, Watanabe K & Fukuchi M
Acclimation of photosynthetic properties in psychrophilic diatom isolated under different light intensities.
Polar Bioscience 13: 43-54 (2000).
Kashino Y, Fujimoto K, Akamatsu A, Koike H, Satoh K & Kudoh S.
Photosynthetic pigment composition of ice algal and phytoplankton assemblages in early spring in Saroma ko lagoon, Hokkaido, Japan.
Proc. NIPR Symp. Polar Biol., 11: pp.22-32 (1998)
If you need contact, please feel free to send e-mail to Kashino.
To send e-mail, please paste @sci.u-hyogo.ac.jp after the mail-account above.